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Inducible expression of Arabidopsis response regulator 22 (ARR22), a type-C ARR, in transgenic Arabidopsis enhances drought and freezing tolerance.

ABSTRACT: The Arabidopsis two-component signaling system, which is comprised of sensor histidine kinases, histidine phosphotransfer proteins, and response regulators, mediates cytokinin response as well as various other plant responses including abiotic stress responses. Arabidopsis response regulators (ARRs) are classified into type-A, -B, and -C. Although the roles of type-A and -B ARRs are well established in Arabidopsis plant signaling, roles of type-C ARRs, ARR22 and ARR24, remain elusive. ARR22, a preferentially cytosolic protein, interacts with certain Arabidopsis histidine phosphotransfer proteins (AHPs) and displays phosphatase activity on AHP5. ARR22 is induced by cold and dehydration. Here, we show that inducible overexpression of ARR22 in Arabidopsis enhanced dehydration, drought, and cold tolerance in a dexamethasone-dependent manner, whereas mutation of the putative phospho-accepting Asp to Asn in ARR22 (ARR22(D74N)) abolished these tolerance phenotypes. Overexpression of ARR22 decreased electrolyte leakage in dehydration-, drought-, or cold-stressed transgenic Arabidopsis plants compared with that of ARR22(D74N) or compared with wild-type plants. Transpiration rates and stomatal apertures were not affected by ARR22 overexpression. No significant difference in both dehydration and freezing tolerance was observed between wild-type and arr22 mutants with or without cytokinin preincubation, consistent with the lack of phenotypes of arr22 mutants in their vegetative development. Meta-profile analyses of the microarray data on ARR22-responsive genes indicate that ARR22 modulates expression of a variety of abiotic stress-responsive genes, which might contribute to increasing drought and freezing tolerance. Taken together, these results suggest that ARR22 plays a positive role in the stress tolerance response in part via enhancing cell membrane integrity and that phospho-histidine phosphatase activity of ARR22 may be required for this function.

SUBMITTER: Kang NY

PROVIDER: S-EPMC3828410 | biostudies-literature | 2013

REPOSITORIES: biostudies-literature

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Inducible expression of Arabidopsis response regulator 22 (ARR22), a type-C ARR, in transgenic Arabidopsis enhances drought and freezing tolerance.

Kang Na Young NY Cho Chuloh C Kim Jungmook J

PloS one 20131114 11

The Arabidopsis two-component signaling system, which is comprised of sensor histidine kinases, histidine phosphotransfer proteins, and response regulators, mediates cytokinin response as well as various other plant responses including abiotic stress responses. Arabidopsis response regulators (ARRs) are classified into type-A, -B, and -C. Although the roles of type-A and -B ARRs are well established in Arabidopsis plant signaling, roles of type-C ARRs, ARR22 and ARR24, remain elusive. ARR22, a p ...[more]

PMID: 24244460

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Project description:NAC (NAM, ATAF, and CUC) transcription factors play important roles in plant biological processes, including phytohormone homeostasis, plant development, and in responses to various environmental stresses.TaNAC29 was introduced into Arabidopsis using the Agrobacterium tumefaciens-mediated floral dipping method. TaNAC29-overexpression plants were subjected to salt and drought stresses for examining gene functions. To investigate tolerant mechanisms involved in the salt and drought responses, expression of related marker genes analyses were conducted, and related physiological indices were also measured. Expressions of genes were analyzed by quantitative real-time polymerase chain reaction (qRT-PCR).A novel NAC transcription factor gene, designated TaNAC29, was isolated from bread wheat (Triticum aestivum). Sequence alignment suggested that TaNAC29 might be located on chromosome 2BS. TaNAC29 was localized to the nucleus in wheat protoplasts, and proved to have transcriptional activation activities in yeast. TaNAC29 was expressed at a higher level in the leaves, and expression levels were much higher in senescent leaves, indicating that TaNAC29 might be involved in the senescence process. TaNAC29 transcripts were increased following treatments with salt, PEG6000, H2O2, and abscisic acid (ABA). To examine TaNAC29 function, transgenic Arabidopsis plants overexpressing TaNAC29 were generated. Germination and root length assays of transgenic plants demonstrated that TaNAC29 overexpression plants had enhanced tolerances to high salinity and dehydration, and exhibited an ABA-hypersensitive response. When grown in the greenhouse, TaNAC29-overexpression plants showed the same tolerance response to salt and drought stresses at both the vegetative and reproductive period, and had delayed bolting and flowering in the reproductive period. Moreover, TaNAC29 overexpression plants accumulated lesser malondialdehyde (MDA), H2O2, while had higher superoxide dismutase (SOD) and catalase (CAT) activities under high salinity and/or dehydration stress.Our results demonstrate that TaNAC29 plays important roles in the senescence process and response to salt and drought stresses. ABA signal pathway and antioxidant enzyme systems are involved in TaNAC29-mediated stress tolerance mechanisms.

Dataset Information

Inducible expression of Arabidopsis response regulator 22 (ARR22), a type-C ARR, in transgenic Arabidopsis enhances drought and freezing tolerance.

Publications

Inducible expression of Arabidopsis response regulator 22 (ARR22), a type-C ARR, in transgenic Arabidopsis enhances drought and freezing tolerance.

Similar Datasets

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