Nucleic acid beacons for long-term real-time intracellular monitoring.
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ABSTRACT:
SUBMITTER: Wu Y
PROVIDER: S-EPMC3830794 | biostudies-literature | 2008 Apr
REPOSITORIES: biostudies-literature
Publications
Nucleic acid beacons for long-term real-time intracellular monitoring.
Wu Yanrong Y Yang Chaoyong James CJ Moroz Leonid L LL Tan Weihong W
Analytical chemistry 20080306 8
Real time intracellular monitoring of biological molecules inside living cells is important in many biomedical studies and reveals valuable information unobtainable by conventional molecular biology techniques. A variety of methods and molecular probes have been developed, but long term (from a few hours to days) intracellular monitoring with high sensitivity and selectivity is impossible and has not been accomplished. We have used locked nucleic acids (LNA) to engineer novel molecular beacons ( ...[more]
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