Unknown

Dataset Information

0

A simple LC-MS/MS method for determination of kynurenine and tryptophan concentrations in human plasma from HIV-infected patients.

ABSTRACT: Indoleamine 2,3-dioxygenase, catalyzing tryptophan (Trp) metabolism through the kynurenine (Kyn) metabolic pathway, plays important roles in immune suppression and the CNS. In this article, we report a simple, rapid and specific LC-MS/MS method for accurate determination of Kyn and Trp concentrations in human plasma from HIV-infected patients.The human plasma sample (100 µl) was mixed with Kyn-d4 and Trp-d5 internal standards and then precipitated with trifluoroacetic acid. The supernatant was directly analyzed by LC-MS/MS. The assay using surrogate matrix calibrators was validated for precision, accuracy, matrix effect, extraction efficiency and stability. Some assay validation issues for endogenous substance bioanalysis using an LC-MS/MS method are discussed.A simple, specific and reproducible LC-MS/MS method has been developed and validated for measuring Kyn and Trp in human plasma samples.

SUBMITTER: Huang Y 

PROVIDER: S-EPMC3830928 | biostudies-literature | 2013 Jun

REPOSITORIES: biostudies-literature

Json Xml
altmetric image

Publications

A simple LC-MS/MS method for determination of kynurenine and tryptophan concentrations in human plasma from HIV-infected patients.

Huang Yong Y   Louie Alexander A   Yang Qiyun Q   Massenkoff Nicholas N   Xu Connie C   Hunt Peter W PW   Gee Winnie W  

Bioanalysis 20130601 11

<h4>Background</h4>Indoleamine 2,3-dioxygenase, catalyzing tryptophan (Trp) metabolism through the kynurenine (Kyn) metabolic pathway, plays important roles in immune suppression and the CNS. In this article, we report a simple, rapid and specific LC-MS/MS method for accurate determination of Kyn and Trp concentrations in human plasma from HIV-infected patients.<h4>Results</h4>The human plasma sample (100 µl) was mixed with Kyn-d4 and Trp-d5 internal standards and then precipitated with trifluor  ...[more]

Similar Datasets

Unknown 4-Vinylpyridine copolymers for improved LC-MS tryptophan and kynurenine determination in human serum.
| S-EPMC11317505 | biostudies-literature
Unknown Rapid Determination of Isomeric Benzoylpaeoniflorin and Benzoylalbiflorin in Rat Plasma by LC-MS/MS Method.
| S-EPMC5439074 | biostudies-literature
Unknown Quantitative method for the determination of iso-fludelone (KOS-1803) in human plasma by LC-MS/MS.
| S-EPMC4179924 | biostudies-literature
Unknown A Simple LC-MS Method for the Quantitation of Alkaloids in Endophyte-Infected Perennial Ryegrass.
| S-EPMC6891275 | biostudies-literature
Unknown A simple, high-throughput and validated LC-MS/MS method for the determination of azithromycin in human plasma and its application to a clinical pharmacokinetic study.
| S-EPMC9539494 | biostudies-literature
Unknown Fast and sensitive LC-MS/MS method for the simultaneous determination of lisinopril and hydrochlorothiazide in human plasma.
| S-EPMC5790688 | biostudies-literature
Unknown A rapid, simple and sensitive LC-MS/MS method for lenvatinib quantification in human plasma for therapeutic drug monitoring.
| S-EPMC8547652 | biostudies-literature
Unknown Validated LC-MS/MS Method for the Determination of Scopoletin in Rat Plasma and Its Application to Pharmacokinetic Studies.
| S-EPMC6332412 | biostudies-literature
Unknown Establishment of LC-MS/MS Method for Determination of GMDTC in Rat Plasma and Its Application in Preclinical Pharmacokinetics.
| S-EPMC9919763 | biostudies-literature
Unknown Simultaneous determination of fludarabine and clofarabine in human plasma by LC-MS/MS.
| S-EPMC4110960 | biostudies-literature