Ontology highlight
ABSTRACT:
SUBMITTER: Zoldak G
PROVIDER: S-EPMC3831443 | biostudies-literature | 2013 Nov
REPOSITORIES: biostudies-literature
Žoldák Gabriel G Stigler Johannes J Pelz Benjamin B Li Hongbin H Rief Matthias M
Proceedings of the National Academy of Sciences of the United States of America 20131021 45
In this study we expand the accessible dynamic range of single-molecule force spectroscopy by optical tweezers to the microsecond range by fast sampling. We are able to investigate a single molecule for up to 15 min and with 300-kHz bandwidth as the protein undergoes tens of millions of folding/unfolding transitions. Using equilibrium analysis and autocorrelation analysis of the time traces, the full energetics as well as real-time kinetics of the ultrafast folding of villin headpiece 35 and a s ...[more]