Ontology highlight
ABSTRACT:
SUBMITTER: Lopez CJ
PROVIDER: S-EPMC3832024 | biostudies-literature | 2013 Nov
REPOSITORIES: biostudies-literature
López Carlos J CJ Yang Zhongyu Z Altenbach Christian C Hubbell Wayne L WL
Proceedings of the National Academy of Sciences of the United States of America 20131028 46
The studies presented here explore the relationship between protein packing and molecular flexibility using ligand-binding cavity mutants of T4 lysozyme. Although previously reported crystal structures of the mutants investigated show single conformations that are similar to the WT protein, site-directed spin labeling in solution reveals additional conformational substates in equilibrium exchange with a WT-like population. Remarkably, binding of ligands, including the general anesthetic halothan ...[more]