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Increased level of E protein activity during invariant NKT development promotes differentiation of invariant NKT2 and invariant NKT17 subsets.

ABSTRACT: E protein transcription factors and their natural inhibitors, Id proteins, play critical and complex roles during lymphoid development. In this article, we report that partial maintenance of E protein activity during positive selection results in a change in the cell fate determination of developing iNKT cells, with a block in the development of iNKT1 cells and a parallel increase in the iNKT2 and iNKT17 subsets. Because the expression levels of the transcription factors that drive these alternative functional fates (GATA-3, ROR?T, T-bet, and Runx-3) are not altered, our results suggest that E protein activity controls a novel checkpoint that regulates the number of iNKT precursors that choose each fate.

SUBMITTER: Hu T

PROVIDER: S-EPMC3832958 | biostudies-literature | 2013 Nov

REPOSITORIES: biostudies-literature

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Increased level of E protein activity during invariant NKT development promotes differentiation of invariant NKT2 and invariant NKT17 subsets.

Hu Taishan T Wang Hongcheng H Simmons Amie A Bajaña Sandra S Zhao Ying Y Kovats Susan S Sun Xiao-Hong XH Alberola-Ila Jose J

Journal of immunology (Baltimore, Md. : 1950) 20131011 10

E protein transcription factors and their natural inhibitors, Id proteins, play critical and complex roles during lymphoid development. In this article, we report that partial maintenance of E protein activity during positive selection results in a change in the cell fate determination of developing iNKT cells, with a block in the development of iNKT1 cells and a parallel increase in the iNKT2 and iNKT17 subsets. Because the expression levels of the transcription factors that drive these alterna ...[more]

PMID: 24123679

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