Multiparametric image analysis of lung-branching morphogenesis.
Ontology highlight
ABSTRACT: Background
Lung-branching morphogenesis is a fundamental developmental process, yet the cellular dynamics that occur during lung development and the molecular mechanisms underlying recent postulated branching modes are poorly understood.Results
Here, we implemented a time-lapse video microscopy method to study the cellular behavior and molecular mechanisms of planar bifurcation and domain branching in lung explant- and organotypic cultures. Our analysis revealed morphologically distinct stages that are shaped at least in part by a combination of localized and orientated cell divisions and by local mechanical forces. We also identified myosin light-chain kinase as an important regulator of bud bifurcation, but not domain branching in lung explants.Conclusions
This live imaging approach provides a method to study cellular behavior during lung-branching morphogenesis and suggests the importance of a mechanism primarily based on oriented cell proliferation and mechanical forces in forming and shaping the developing lung airways.
SUBMITTER: Schnatwinkel C
PROVIDER: S-EPMC3833092 | biostudies-literature | 2013 Jun
REPOSITORIES: biostudies-literature
Publications
Multiparametric image analysis of lung-branching morphogenesis.
Schnatwinkel Carsten C Niswander Lee L
Developmental dynamics : an official publication of the American Association of Anatomists 20130423 6
<h4>Background</h4>Lung-branching morphogenesis is a fundamental developmental process, yet the cellular dynamics that occur during lung development and the molecular mechanisms underlying recent postulated branching modes are poorly understood.<h4>Results</h4>Here, we implemented a time-lapse video microscopy method to study the cellular behavior and molecular mechanisms of planar bifurcation and domain branching in lung explant- and organotypic cultures. Our analysis revealed morphologically d ...[more]
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