Fine tuning and efficient T cell activation with stimulatory aCD3 nanoarrays.
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ABSTRACT:
SUBMITTER: Matic J
PROVIDER: S-EPMC3834297 | biostudies-literature | 2013 Nov
REPOSITORIES: biostudies-literature
Publications
Fine tuning and efficient T cell activation with stimulatory aCD3 nanoarrays.
Matic Jovana J Deeg Janosch J Scheffold Alexander A Goldstein Itamar I Spatz Joachim P JP
Nano letters 20131017 11
Anti-CD3 (aCD3) nanoarrays fabricated by self-assembled nanopatterning combined with site-directed protein immobilization techniques represent a novel T cell stimulatory platform that allows tight control over ligand orientation and surface density. Here, we show that activation of primary human CD4+ T cells, defined by CD69 upregulation, IL-2 production and cell proliferation, correlates with aCD3 density on nanoarrays. Immobilization of aCD3 through nanopatterning had two effects: cell activat ...[more]
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