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Enhanced Electrostatic Discrimination of Proteins on Nanoparticle-Coated Surfaces.

ABSTRACT: Two ?-lactoglobulin (BLG) isoforms, BLGA and BLGB, were used a test bed for the differentiation of proteins using electrostatics. In these studies, the BLGA and BLGB binding to a highly charged, cationic gold nanoparticle (GNP) modified surface was investigated by atomic force microscopy (AFM) and surface plasmon resonance (SPR) spectroscopy The binding affinity, and more importantly, the selectivity of this surface towards these two almost identical protein isoforms were both significantly increased on the cationic GNP surface array relative to the values measured with the same free cationic GNP in solution. While protein recognition is traditionally achieved almost exclusively via orientation dependent short-range interactions such as hydrogen bonds and hydrophobic interactions, our results show the potential of protein recognition platforms based on enhanced electrostatic interactions.

SUBMITTER: Xu Y

PROVIDER: S-EPMC3835305 | biostudies-literature | 2013 Oct

REPOSITORIES: biostudies-literature

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Enhanced Electrostatic Discrimination of Proteins on Nanoparticle-Coated Surfaces.

Xu Yisheng Y Engel Yoni Y Yan Yunfeng Y Chen Kaimin K Moyano Daniel F DF Dubin Paul L PL Rotello Vincent M VM

Journal of materials chemistry. B 20131001 39

Two β-lactoglobulin (BLG) isoforms, BLGA and BLGB, were used a test bed for the differentiation of proteins using electrostatics. In these studies, the BLGA and BLGB binding to a highly charged, cationic gold nanoparticle (GNP) modified surface was investigated by atomic force microscopy (AFM) and surface plasmon resonance (SPR) spectroscopy The binding affinity, and more importantly, the selectivity of this surface towards these two almost identical protein isoforms were both significantly incr ...[more]

PMID: 24273645

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