Total chemical synthesis of human proinsulin.
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ABSTRACT:
SUBMITTER: Luisier S
PROVIDER: S-EPMC3835593 | biostudies-literature | 2010 Nov
REPOSITORIES: biostudies-literature
Publications
Total chemical synthesis of human proinsulin.
Luisier Samuel S Avital-Shmilovici Michal M Weiss Michael A MA Kent Stephen B H SB
Chemical communications (Cambridge, England) 20100928 43
A convergent synthetic strategy based on modern chemical ligation methods was used to make human proinsulin. The synthetic protein was characterized by LCMS, CD spectroscopy, and by 1D- and 2D-NMR spectroscopy. Synthetic human proinsulin had full biochemical activity in a receptor-binding assay. ...[more]
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