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Methylome analysis and epigenetic changes associated with menarcheal age.


ABSTRACT: Reproductive factors have been linked to both breast cancer and DNA methylation, suggesting methylation as an important mechanism by which reproductive factors impact on disease risk. However, few studies have investigated the link between reproductive factors and DNA methylation in humans. Genome-wide methylation in peripheral blood lymphocytes of 376 healthy women from the prospective EPIC study was investigated using LUminometric Methylation Assay (LUMA). Also, methylation of 458877 CpG sites was additionally investigated in an independent group of 332 participants of the EPIC-Italy sub-cohort, using the Infinium HumanMethylation 450 BeadChip. Multivariate logistic regression and linear models were used to investigate the association between reproductive risk factors and genome wide and CpG-specific DNA methylation, respectively. Menarcheal age was inversely associated with global DNA methylation as measured with LUMA. For each yearly increase in age at menarche, the risk of having genome wide methylation below median level was increased by 32% (OR:1.32, 95%CI:1.14-1.53). When age at menarche was treated as a categorical variable, there was an inverse dose-response relationship with LUMA methylation levels (OR(12-14 vs. ?11 yrs):1.78, 95%CI:1.01-3.17 and OR(?15 vs. ?11 yrs):4.59, 95%CI:2.04-10.33; P for trend<0.0001). However, average levels of global methylation as measured by the Illumina technology were not significantly associated with menarcheal age. In locus by locus comparative analyses, only one CpG site had significantly different methylation depending on the menarcheal age category examined, but this finding was not replicated by pyrosequencing in an independent data set. This study suggests a link between age at menarche and genome wide DNA methylation, and the difference in results between the two arrays suggests that repetitive element methylation has a role in the association. Epigenetic changes may be modulated by menarcheal age, or the association may be a mirror of other important changes in early life that have a detectable effect on both methylation levels and menarcheal age.

SUBMITTER: Demetriou CA 

PROVIDER: S-EPMC3835804 | biostudies-literature | 2013

REPOSITORIES: biostudies-literature

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Methylome analysis and epigenetic changes associated with menarcheal age.

Demetriou Christiana A CA   Chen Jia J   Polidoro Silvia S   van Veldhoven Karin K   Cuenin Cyrille C   Campanella Gianluca G   Brennan Kevin K   Clavel-Chapelon Françoise F   Dossus Laure L   Kvaskoff Marina M   Drogan Dagmar D   Boeing Heiner H   Kaaks Rudolf R   Risch Angela A   Trichopoulos Dimitrios D   Lagiou Pagona P   Masala Giovanna G   Sieri Sabina S   Tumino Rosario R   Panico Salvatore S   Quirós J Ramón JR   Sánchez Perez María-José MJ   Amiano Pilar P   Huerta Castaño José María JM   Ardanaz Eva E   Onland-Moret Charlotte C   Peeters Petra P   Khaw Kay-Tee KT   Wareham Nick N   Key Timothy J TJ   Travis Ruth C RC   Romieu Isabelle I   Gallo Valentina V   Gunter Marc M   Herceg Zdenko Z   Kyriacou Kyriacos K   Riboli Elio E   Flanagan James M JM   Vineis Paolo P  

PloS one 20131120 11


Reproductive factors have been linked to both breast cancer and DNA methylation, suggesting methylation as an important mechanism by which reproductive factors impact on disease risk. However, few studies have investigated the link between reproductive factors and DNA methylation in humans. Genome-wide methylation in peripheral blood lymphocytes of 376 healthy women from the prospective EPIC study was investigated using LUminometric Methylation Assay (LUMA). Also, methylation of 458877 CpG sites  ...[more]

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