Ontology highlight
ABSTRACT:
SUBMITTER: Taylor SM
PROVIDER: S-EPMC3836461 | biostudies-literature | 2013 Dec
REPOSITORIES: biostudies-literature
The Journal of infectious diseases 20130801 12
Molecular surveillance for drug-resistant malaria parasites requires reliable, timely, and scalable methods. These data may be efficiently produced by genotyping parasite populations using second-generation sequencing (SGS). We designed and validated a SGS protocol to quantify mutant allele frequencies in the Plasmodium falciparum genes dhfr and dhps in mixed isolates. We applied this new protocol to field isolates from children and compared it to standard genotyping using Sanger sequencing. The ...[more]