Molecular diagnosis of Actinomadura madurae infection by 16S rRNA deep sequencing.
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ABSTRACT:
SUBMITTER: Salipante SJ
PROVIDER: S-EPMC3838028 | biostudies-literature | 2013 Dec
REPOSITORIES: biostudies-literature
Publications
Molecular diagnosis of Actinomadura madurae infection by 16S rRNA deep sequencing.
Salipante Stephen J SJ Sengupta Dhruba J DJ Hoogestraat Daniel R DR Cummings Lisa A LA Bryant Bronwyn H BH Natividad Catherine C Thielges Stephanie S Monsaas Peter W PW Chau Mimosa M Barbee Lindley A LA Rosenthal Christopher C Cookson Brad T BT Hoffman Noah G NG
Journal of clinical microbiology 20131009 12
Next-generation DNA sequencing can be used to catalog individual organisms within complex, polymicrobial specimens. Here, we utilized deep sequencing of 16S rRNA to implicate Actinomadura madurae as the cause of mycetoma in a diabetic patient when culture and conventional molecular methods were overwhelmed by overgrowth of other organisms. ...[more]
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