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Genetic marker suitable for identification and genotyping of Plasmodium ovale curtisi and Plasmodium ovale wallikeri.


ABSTRACT: We present a seminested PCR method that specifically discriminates between Plasmodium ovale curtisi and P. ovale wallikeri with high sensitivity. The test is based on species-specific amplification of a size-polymorphic fragment of the tryptophan-rich antigen gene, potra, which also permits discrimination of intraspecific sequence variants at this locus.

SUBMITTER: Tanomsing N 

PROVIDER: S-EPMC3838052 | biostudies-literature | 2013 Dec

REPOSITORIES: biostudies-literature

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Genetic marker suitable for identification and genotyping of Plasmodium ovale curtisi and Plasmodium ovale wallikeri.

Tanomsing Naowarat N   Imwong Mallika M   Sutherland Colin J CJ   Dolecek Christiane C   Hien Tran Tinh TT   Nosten Francois F   Day Nicholas P J NP   White Nicholas J NJ   Snounou Georges G  

Journal of clinical microbiology 20130925 12


We present a seminested PCR method that specifically discriminates between Plasmodium ovale curtisi and P. ovale wallikeri with high sensitivity. The test is based on species-specific amplification of a size-polymorphic fragment of the tryptophan-rich antigen gene, potra, which also permits discrimination of intraspecific sequence variants at this locus. ...[more]

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