Detection of heteroresistant Mycobacterium tuberculosis by pyrosequencing.
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ABSTRACT:
SUBMITTER: Engstrom A
PROVIDER: S-EPMC3838067 | biostudies-literature | 2013 Dec
REPOSITORIES: biostudies-literature
Publications
Detection of heteroresistant Mycobacterium tuberculosis by pyrosequencing.
Engström Anna A Hoffner Sven S Juréen Pontus P
Journal of clinical microbiology 20130918 12
The ability of pyrosequencing to detect a resistant minority population of a heteroresistant Mycobacterium tuberculosis strain was investigated by performing a titration study. A mutant signal was noted only when the proportion of mutant DNA in the DNA target was 35 to 50%, showing that the sensitivity is significantly lower than that of phenotypic drug susceptibility test methods. ...[more]
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