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Mapping three-dimensional temperature in microfluidic chip.

ABSTRACT: Three-dimensional (3D) temperature mapping method with high spatial resolution and acquisition rate is of vital importance in evaluating thermal processes in micro-environment. We have synthesized a new temperature-sensitive functional material (Rhodamine B functionalized Polydimethylsiloxane). By performing optical sectioning of this material, we established an advanced method for visualizing the micro-scale 3D thermal distribution inside microfluidic chip with down to 10 ms temporal resolution and 2 ~ 6 °C temperature resolution depending the capture parameters. This method is successfully applied to monitor the local temperature variation throughout micro-droplet heat transfer process and further reveal exothermic nanoliter droplet reactions to be unique and milder than bench-top experiment.

SUBMITTER: Wu J

PROVIDER: S-EPMC3839638 | biostudies-literature | 2013 Nov

REPOSITORIES: biostudies-literature

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Mapping three-dimensional temperature in microfluidic chip.

Wu Jinbo J Kwok Tsz Yan TY Li Xiaolin X Cao Wenbin W Wang Yu Y Huang Junying J Hong Yaying Y Zhang Dongen D Wen Weijia W

Scientific reports 20131125

Three-dimensional (3D) temperature mapping method with high spatial resolution and acquisition rate is of vital importance in evaluating thermal processes in micro-environment. We have synthesized a new temperature-sensitive functional material (Rhodamine B functionalized Polydimethylsiloxane). By performing optical sectioning of this material, we established an advanced method for visualizing the micro-scale 3D thermal distribution inside microfluidic chip with down to 10 ms temporal resolution ...[more]

PMID: 24276475

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Project description:Study questionCan we reconstitute physiologically relevant 3-dimensional (3D) microengineered endometrium in-vitro model?Summary answerOur representative microengineered vascularised endometrium on-a-chip closely recapitulates the endometrial microenvironment that consists of three distinct layers including epithelial cells, stromal fibroblasts and endothelial cells in a 3D extracellular matrix in a spatiotemporal manner.What is known alreadyOrgan-on-a-chip, a multi-channel 3D microfluidic cell culture system, is widely used to investigate physiologically relevant responses of organ systems.Study design, size, durationThe device consists of five microchannels that are arrayed in parallel and partitioned by array of micropost. Two central channels are for 3D culture and morphogenesis of stromal fibroblast and endothelial cells. In addition, the outermost channel is for the culture of additional endometrial stromal fibroblasts that secrete biochemical cues to induce directional pro-angiogenic responses of endothelial cells. To seed endometrial epithelial cells, on Day 8, Ishikawa cells were introduced to one of the two medium channels to adhere on the gel surface. After that, the microengineered endometrium was cultured for an additional 5-6 days (total ∼ 14 days) for the purpose of each experiment.Participants/materials, setting, methodsMicrofluidic 3D cultures were maintained in endothelial growth Medium 2 with or without oestradiol and progesterone. Some cultures additionally received exogenous pro-angiogenic factors. For the three distinct layers of microengineered endometrium-on-a-chip, the epithelium, stroma and blood vessel characteristics and drug response of each distinct layer in the microfluidic model were assessed morphologically and biochemically. The quantitative measurement of endometrial drug delivery was evaluated by the permeability coefficients.Main results and the role of chanceWe established microengineered vascularised endometrium-on-chip, which consists of three distinct layers: epithelium, stroma and blood vessels. Our endometrium model faithfully recapitulates in-vivo endometrial vasculo-angiogenesis and hormonal responses displaying key features of the proliferative and secretory phases of the menstrual cycle. Furthermore, the effect of the emergency contraception drug levonorgestrel was evaluated in our model demonstrating increased endometrial permeability and blood vessel regression in a dose-dependent manner. We finally provided a proof of concept of the multi-layered endometrium model for embryo implantation, which aids a better understanding of the molecular and cellular mechanisms underlying this process.Large scale dataN/A.Limitations, reasons for cautionThis report is largely an in-vitro study and it would be beneficial to validate our findings using human primary endometrial cells.Wider implications of the findingsOur 3D microengineered vascularised endometrium-on-a-chip provides a new in-vitro approach to drug screening and drug discovery by mimicking the complicated behaviours of human endometrium. Thus, we suggest our model as a tool for addressing critical challenges and unsolved problems in female diseases, such as endometriosis, uterine cancer and female infertility, in a personalised manner.Study funding/competing interest(s)This work is supported by funding from the National Research Foundation of Korea (NRF) grant funded by the Korea government (MSIT) to Y.J.K. (No. 2018R1C1B6003), to J.A. (No. 2020R1I1A1A01074136) and to H.S.K. (No. 2020R1C1C100787212). The authors report no conflicts of interest.

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Mapping three-dimensional temperature in microfluidic chip.

Publications

Mapping three-dimensional temperature in microfluidic chip.

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