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Structural basis of substrate conversion in a new aromatic peroxygenase: cytochrome P450 functionality with benefits.


ABSTRACT: Aromatic peroxygenases (APOs) represent a unique oxidoreductase sub-subclass of heme proteins with peroxygenase and peroxidase activity and were thus recently assigned a distinct EC classification (EC 1.11.2.1). They catalyze, inter alia, oxyfunctionalization reactions of aromatic and aliphatic hydrocarbons with remarkable regio- and stereoselectivities. When compared with cytochrome P450, APOs appear to be the choice enzymes for oxyfunctionalizations in organic synthesis due to their independence from a cellular environment and their greater chemical versatility. Here, the first two crystal structures of a heavily glycosylated fungal aromatic peroxygenase (AaeAPO) are described. They reveal different pH-dependent ligand binding modes. We model the fitting of various substrates in AaeAPO, illustrating the way the enzyme oxygenates polycyclic aromatic hydrocarbons. Spatial restrictions by a phenylalanine pentad in the active-site environment govern substrate specificity in AaeAPO.

SUBMITTER: Piontek K 

PROVIDER: S-EPMC3843090 | biostudies-literature | 2013 Nov

REPOSITORIES: biostudies-literature

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Structural basis of substrate conversion in a new aromatic peroxygenase: cytochrome P450 functionality with benefits.

Piontek Klaus K   Strittmatter Eric E   Ullrich René R   Gröbe Glenn G   Pecyna Marek J MJ   Kluge Martin M   Scheibner Katrin K   Hofrichter Martin M   Plattner Dietmar A DA  

The Journal of biological chemistry 20131014 48


Aromatic peroxygenases (APOs) represent a unique oxidoreductase sub-subclass of heme proteins with peroxygenase and peroxidase activity and were thus recently assigned a distinct EC classification (EC 1.11.2.1). They catalyze, inter alia, oxyfunctionalization reactions of aromatic and aliphatic hydrocarbons with remarkable regio- and stereoselectivities. When compared with cytochrome P450, APOs appear to be the choice enzymes for oxyfunctionalizations in organic synthesis due to their independen  ...[more]

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