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Human papillomavirus (HPV) origin-binding protein associates with mitotic spindles to enable viral DNA partitioning.

ABSTRACT: Human papillomaviruses (HPVs) establish long-term infections in patients. The mechanism for extrachromosomal HPV DNA persistence in cycling cells is unknown. We show that HPV origin-containing plasmids partition as minichromosomes, attributable to an association of the viral origin recognition protein E2 with mitotic spindles. alpha-, beta-, and gamma-tubulins were pulled down with a tagged E2. The N-terminal transacting and C-terminal protein dimerization/DNA binding domains independently associated with the spindles. We suggest that this E2 property enables these viruses to establish persistence. Its implication for HPV oncogenesis is presented.

SUBMITTER: Van Tine BA

PROVIDER: S-EPMC384690 | biostudies-literature | 2004 Mar

REPOSITORIES: biostudies-literature

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Human papillomavirus (HPV) origin-binding protein associates with mitotic spindles to enable viral DNA partitioning.

Van Tine Brian A BA Dao Luan D LD Wu Shwu-Yuan SY Sonbuchner Timothy M TM Lin Biing Yuan BY Zou Nianxiang N Chiang Cheng-Ming CM Broker Thomas R TR Chow Louise T LT

Proceedings of the National Academy of Sciences of the United States of America 20040312 12

Human papillomaviruses (HPVs) establish long-term infections in patients. The mechanism for extrachromosomal HPV DNA persistence in cycling cells is unknown. We show that HPV origin-containing plasmids partition as minichromosomes, attributable to an association of the viral origin recognition protein E2 with mitotic spindles. alpha-, beta-, and gamma-tubulins were pulled down with a tagged E2. The N-terminal transacting and C-terminal protein dimerization/DNA binding domains independently assoc ...[more]

PMID: 15020762

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