Ontology highlight
ABSTRACT:
SUBMITTER: Wang Y
PROVIDER: S-EPMC3847046 | biostudies-literature | 2013
REPOSITORIES: biostudies-literature
Wang Yueying Y Peng Donghai D Dong Zhaoxia Z Zhu Lei L Guo Suxia S Sun Ming M
PloS one 20131202 12
In this study, we report a rapid cloning strategy for large native plasmids via a contig linkage map by BAC libraries. Using this method, we cloned a large plasmid pBMB165 from Bacillus thuringiensis serovar tenebrionis strain YBT-1765. Complete sequencing showed that pBMB165 is 77,627 bp long with a GC-content of 35.36%, and contains 103 open reading frames (ORFs). Sequence analysis and comparison reveals that pBMB165 represents a novel plasmid organization: it mainly consists of a pXO2-like re ...[more]