High resolution melting analysis: rapid and precise characterisation of recombinant influenza A genomes.
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ABSTRACT:
SUBMITTER: Kalthoff D
PROVIDER: S-EPMC3847122 | biostudies-literature | 2013
REPOSITORIES: biostudies-literature
Publications
High resolution melting analysis: rapid and precise characterisation of recombinant influenza A genomes.
Kalthoff Donata D Beer Martin M Hoffmann Bernd B
Virology journal 20130912
<h4>Background</h4>High resolution melting analysis (HRM) is a rapid and cost-effective technique for the characterisation of PCR amplicons. Because the reverse genetics of segmented influenza A viruses allows the generation of numerous influenza A virus reassortants within a short time, methods for the rapid selection of the correct recombinants are very useful.<h4>Methods</h4>PCR primer pairs covering the single nucleotide polymorphism (SNP) positions of two different influenza A H5N1 strains ...[more]
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