Ontology highlight
ABSTRACT:
SUBMITTER: Simmons G
PROVIDER: S-EPMC384725 | biostudies-literature | 2004 Mar
REPOSITORIES: biostudies-literature
Simmons Graham G Reeves Jacqueline D JD Rennekamp Andrew J AJ Amberg Sean M SM Piefer Andrew J AJ Bates Paul P
Proceedings of the National Academy of Sciences of the United States of America 20040309 12
Severe acute respiratory syndrome-associated coronavirus (SARS-CoV) is a rapidly emerging pathogen with potentially serious consequences for public health. Here we describe conditions that result not only in the efficient expression of the SARS-CoV spike (S) protein on the surface of cells, but in its incorporation into lentiviral particles that can be used to transduce cells in an S glycoprotein-dependent manner. We found that although some primate cell lines, including Vero E6, 293T and Huh-7 ...[more]