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Proteomic mapping provides powerful insights into functional myelin biology.

ABSTRACT: Myelin is a dynamic, functionally active membrane necessary for rapid action potential conduction, axon survival, and cytoarchitecture. The number of debilitating neurological disorders that occur when myelin is disrupted emphasizes its importance. Using high-resolution 2D gel electrophoresis, mass spectrometry, and immunoblotting, we have developed an extensive proteomic map of proteins present in myelin, identifying 98 proteins corresponding to at least 130 of the approximately 200 spots on the map. This proteomic map has been applied to analyses of the localization and function of selected proteins, providing a powerful tool to investigate the diverse functions of myelin.

SUBMITTER: Taylor CM

PROVIDER: S-EPMC384800 | biostudies-literature | 2004 Mar

REPOSITORIES: biostudies-literature

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Proteomic mapping provides powerful insights into functional myelin biology.

Taylor Christopher M CM Marta Cecilia B CB Claycomb Robert J RJ Han David K DK Rasband Matthew N MN Coetzee Timothy T Pfeiffer Steven E SE

Proceedings of the National Academy of Sciences of the United States of America 20040319 13

Myelin is a dynamic, functionally active membrane necessary for rapid action potential conduction, axon survival, and cytoarchitecture. The number of debilitating neurological disorders that occur when myelin is disrupted emphasizes its importance. Using high-resolution 2D gel electrophoresis, mass spectrometry, and immunoblotting, we have developed an extensive proteomic map of proteins present in myelin, identifying 98 proteins corresponding to at least 130 of the approximately 200 spots on th ...[more]

PMID: 15070771

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