Ontology highlight
ABSTRACT:
SUBMITTER: Buranda T
PROVIDER: S-EPMC3848308 | biostudies-literature | 2013 Nov
REPOSITORIES: biostudies-literature
Buranda Tione T BasuRay Soumik S Swanson Scarlett S Agola Jacob J Bondu Virginie V Wandinger-Ness Angela A
Analytical biochemistry 20130806 2
We describe a rapid assay for measuring the cellular activity of small guanine triphosphatases (GTPases) in response to a specific stimulus. Effector-functionalized beads are used to quantify in parallel multiple GTP-bound GTPases in the same cell lysate by flow cytometry. In a biologically relevant example, five different Ras family GTPases are shown for the first time to be involved in a concerted signaling cascade downstream of receptor ligation by Sin Nombre hantavirus. ...[more]