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Growth profile of Carboxydothermus hydrogenoformans on pyruvate.

ABSTRACT: Carboxydothermus hydrogenoformans is a thermophilic anaerobic strain most widely known for its ability to produce hydrogen (H2) when grown on carbon monoxide (CO). Although relatively well studied, growth characterization on pyruvate has never been assessed. The present work fully characterizes growth of the bacterium on pyruvate as a sole carbon source. C. hydrogenoformans demonstrated a growth rate of 0.03 h-1, with pyruvate consumption ranging between 0.21 and 0.48 mol?·?g-1 volatile suspended solid?·?d-1. A lag phase was also observed when switching from pyruvate to CO. When grown simultaneously on pyruvate and CO, pyruvate consumption was initiated upon CO depletion. This was attributed to pyruvate oxidation inhibition by CO, and not to a diauxic phenomenom. The strain also showed homoacetogenic activity.

SUBMITTER: Haddad M

PROVIDER: S-EPMC3851474 | biostudies-literature | 2013 Oct

REPOSITORIES: biostudies-literature

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Growth profile of Carboxydothermus hydrogenoformans on pyruvate.

Haddad Mathieu M Cimpoia Ruxandra R Zhao Ya Y Guiot Serge R SR

AMB Express 20131007 1

Carboxydothermus hydrogenoformans is a thermophilic anaerobic strain most widely known for its ability to produce hydrogen (H2) when grown on carbon monoxide (CO). Although relatively well studied, growth characterization on pyruvate has never been assessed. The present work fully characterizes growth of the bacterium on pyruvate as a sole carbon source. C. hydrogenoformans demonstrated a growth rate of 0.03 h-1, with pyruvate consumption ranging between 0.21 and 0.48 mol · g-1 volatile suspende ...[more]

PMID: 24099169

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Carboxydothermus hydrogenoformans

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Project description:Hyperthermophilic bacterium that can grow on carbon monoxide

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A functional Ni-Ni-[4Fe-4S] cluster in the monomeric acetyl-CoA synthase from Carboxydothermus hydrogenoformans.

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A 'Split-Gene' Transketolase From the Hyper-Thermophilic Bacterium <i>Carboxydothermus hydrogenoformans</i>: Structure and Biochemical Characterization.

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