Crystal structure of the cataract-causing P23T ?D-crystallin mutant.
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ABSTRACT:
SUBMITTER: Ji F
PROVIDER: S-EPMC3852438 | biostudies-literature | 2013 Sep
REPOSITORIES: biostudies-literature
Publications
Crystal structure of the cataract-causing P23T γD-crystallin mutant.
Ji Fangling F Koharudin Leonardus M I LM Jung Jinwon J Gronenborn Angela M AM
Proteins 20130617 9
Up to now, efforts to crystallize the cataract-associated P23T mutant of human γD-crystallin have not been successful. Therefore, insights into the light scattering mechanism of this mutant have been exclusively obtained from solution work. Here we present the first crystal structure of the P23T mutant at 2.5 Å resolution. The protein exhibits essentially the same overall structure as seen for the wild-type protein. Based on our structural data, we confirm that no major conformational changes ar ...[more]
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