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Detection of Leptospira-specific antibodies using a recombinant antigen-based enzyme-linked immunosorbent assay.

ABSTRACT: We produced three highly purified recombinant antigens rLipL32, rLipL41, and rLigA-Rep (leptospiral immunoglobulin-like A repeat region) for the detection of Leptospira-specific antibodies in an enzyme-linked immunosorbent assay (ELISA). The performance of these recombinant antigens was evaluated using 121 human sera. Among them, 63 sera were microscopic agglutination test (MAT)-confirmed positive sera from febrile patients in Peru, 22 sera were indigenous MAT-negative febrile patient sera, and 36 sera were from patients with other febrile diseases from Southeast Asia, where leptospirosis is also endemic. Combining the results of immunoglobulin M (IgM) and IgG detection from these three antigens, the overall sensitivity is close to 90% based on the MAT. These results suggest that an ELISA using multiple recombinant antigens may be used as an alternative method for the detection of Leptospira-specific antibodies.

SUBMITTER: Chen HW 

PROVIDER: S-EPMC3854885 | biostudies-literature | 2013 Dec

REPOSITORIES: biostudies-literature

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Detection of Leptospira-specific antibodies using a recombinant antigen-based enzyme-linked immunosorbent assay.

Chen Hua-Wei HW   Zhang Zhiwen Z   Halsey Eric S ES   Guevara Carolina C   Canal Enrique E   Hall Eric E   Maves Ryan R   Tilley Drake H DH   Kochel Tadeusz J TJ   Ching Wei-Mei WM  

The American journal of tropical medicine and hygiene 20131028 6

We produced three highly purified recombinant antigens rLipL32, rLipL41, and rLigA-Rep (leptospiral immunoglobulin-like A repeat region) for the detection of Leptospira-specific antibodies in an enzyme-linked immunosorbent assay (ELISA). The performance of these recombinant antigens was evaluated using 121 human sera. Among them, 63 sera were microscopic agglutination test (MAT)-confirmed positive sera from febrile patients in Peru, 22 sera were indigenous MAT-negative febrile patient sera, and  ...[more]

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