Unknown

Dataset Information

0

Architectural niche organization by LHX2 is linked to hair follicle stem cell function.


ABSTRACT: In adult skin, self-renewing, undifferentiated hair follicle stem cells (HF-SCs) reside within a specialized niche, where they spend prolonged times as a single layer of polarized, quiescent epithelial cells. When sufficient activating signals accumulate, HF-SCs become mobilized to fuel tissue regeneration and hair growth. Here, we show that architectural organization of the HF-SC niche by transcription factor LHX2 plays a critical role in HF-SC behavior. Using genome-wide chromatin and transcriptional profiling of HF-SCs in vivo, we show that LHX2 directly transactivates genes that orchestrate cytoskeletal dynamics and adhesion. Conditional ablation of LHX2 results in gross cellular disorganization and HF-SC polarization within the niche. LHX2 loss leads to a failure to maintain HF-SC quiescence and hair anchoring, as well as progressive transformation of the niche into a sebaceous gland. These findings suggest that niche organization underlies the requirement for LHX2 in hair follicle structure and function.

SUBMITTER: Folgueras AR 

PROVIDER: S-EPMC3856689 | biostudies-literature | 2013 Sep

REPOSITORIES: biostudies-literature

altmetric image

Publications

Architectural niche organization by LHX2 is linked to hair follicle stem cell function.

Folgueras Alicia R AR   Guo Xingyi X   Pasolli H Amalia HA   Stokes Nicole N   Polak Lisa L   Zheng Deyou D   Fuchs Elaine E  

Cell stem cell 20130901 3


In adult skin, self-renewing, undifferentiated hair follicle stem cells (HF-SCs) reside within a specialized niche, where they spend prolonged times as a single layer of polarized, quiescent epithelial cells. When sufficient activating signals accumulate, HF-SCs become mobilized to fuel tissue regeneration and hair growth. Here, we show that architectural organization of the HF-SC niche by transcription factor LHX2 plays a critical role in HF-SC behavior. Using genome-wide chromatin and transcri  ...[more]

Similar Datasets

2013-09-05 | E-GEOD-48068 | biostudies-arrayexpress
2013-09-05 | GSE48068 | GEO
| S-EPMC7073016 | biostudies-literature
| S-EPMC4454174 | biostudies-other
| S-EPMC4457634 | biostudies-literature
| S-EPMC3056115 | biostudies-literature
| S-EPMC7540726 | biostudies-literature
| S-EPMC4640969 | biostudies-literature
| S-EPMC3690395 | biostudies-literature
| S-EPMC3050564 | biostudies-literature