Ontology highlight
ABSTRACT:
SUBMITTER: Bae B
PROVIDER: S-EPMC3856789 | biostudies-literature | 2013 Dec
REPOSITORIES: biostudies-literature
Bae Brian B Davis Elizabeth E Brown Daniel D Campbell Elizabeth A EA Wigneshweraraj Sivaramesh S Darst Seth A SA
Proceedings of the National Academy of Sciences of the United States of America 20131111 49
Bacteriophage T7 encodes an essential inhibitor of the Escherichia coli host RNA polymerase (RNAP), the product of gene 2 (Gp2). We determined a series of X-ray crystal structures of E. coli RNAP holoenzyme with or without Gp2. The results define the structure and location of the RNAP σ(70) subunit domain 1.1(σ(1.1)(70)) inside the RNAP active site channel, where it must be displaced by the DNA upon formation of the open promoter complex. The structures and associated data, combined with previou ...[more]