Ontology highlight
ABSTRACT:
SUBMITTER: Grindberg RV
PROVIDER: S-EPMC3856806 | biostudies-literature | 2013 Dec
REPOSITORIES: biostudies-literature
Grindberg Rashel V RV Yee-Greenbaum Joyclyn L JL McConnell Michael J MJ Novotny Mark M O'Shaughnessy Andy L AL Lambert Georgina M GM Araúzo-Bravo Marcos J MJ Lee Jun J Fishman Max M Robbins Gillian E GE Lin Xiaoying X Venepally Pratap P Badger Jonathan H JH Galbraith David W DW Gage Fred H FH Lasken Roger S RS
Proceedings of the National Academy of Sciences of the United States of America 20131118 49
It has recently been established that synthesis of double-stranded cDNA can be done from a single cell for use in DNA sequencing. Global gene expression can be quantified from the number of reads mapping to each gene, and mutations and mRNA splicing variants determined from the sequence reads. Here we demonstrate that this method of transcriptomic analysis can be done using the extremely low levels of mRNA in a single nucleus, isolated from a mouse neural progenitor cell line and from dissected ...[more]