Infrared laser pulse triggers increased singlet oxygen production in tumour cells.

ABSTRACT: Photodynamic therapy (PDT) is a technique developed to treat the ever-increasing global incidence of cancer. This technique utilises singlet oxygen ((1)O2) generation via a laser excited photosensitiser (PS) to kill cancer cells. However, prolonged sensitivity to intensive light (6-8 weeks for lung cancer), relatively low tissue penetration by activating light (630?nm up to 4?mm), and the cost of PS administration can limit progressive PDT applications. The development of quantum-dot laser diodes emitting in the highest absorption region (1268?nm) of triplet oxygen ((3)O2) presents the possibility of inducing apoptosis in tumour cells through direct (3)O2 ? (1)O2 transition. Here we demonstrate that a single laser pulse triggers dose-dependent (1)O2 generation in both normal keratinocytes and tumour cells and show that tumour cells yield the highest (1)O2 far beyond the initial laser pulse exposure. Our modelling and experimental results support the development of direct infrared (IR) laser-induced tumour treatment as a promising approach in tumour PDT.

SUBMITTER: Sokolovski SG 

PROVIDER: S-EPMC3860013 | biostudies-literature | 2013 Dec

REPOSITORIES: biostudies-literature