Dataset Information

A microbiota signature associated with experimental food allergy promotes allergic sensitization and anaphylaxis.

ABSTRACT:

Background

Commensal microbiota play a critical role in maintaining oral tolerance. The effect of food allergy on the gut microbial ecology remains unknown.

Objective

We sought to establish the composition of the gut microbiota in experimental food allergy and its role in disease pathogenesis.

Methods

Food allergy-prone mice with a gain-of-function mutation in the IL-4 receptor ? chain (Il4raF709) and wild-type (WT) control animals were subjected to oral sensitization with chicken egg ovalbumin (OVA). Enforced tolerance was achieved by using allergen-specific regulatory T (Treg) cells. Community structure analysis of gut microbiota was performed by using a high-density 16S rDNA oligonucleotide microarrays (PhyloChip) and massively parallel pyrosequencing of 16S rDNA amplicons.

Results

OVA-sensitized Il4raF709 mice exhibited a specific microbiota signature characterized by coordinate changes in the abundance of taxa of several bacterial families, including the Lachnospiraceae, Lactobacillaceae, Rikenellaceae, and Porphyromonadaceae. This signature was not shared by similarly sensitized WT mice, which did not exhibit an OVA-induced allergic response. Treatment of OVA-sensitized Il4raF709 mice with OVA-specific Treg cells led to a distinct tolerance-associated signature coincident with the suppression of the allergic response. The microbiota of allergen-sensitized Il4raF709 mice differentially promoted OVA-specific IgE responses and anaphylaxis when reconstituted in WT germ-free mice.

Conclusion

Mice with food allergy exhibit a specific gut microbiota signature capable of transmitting disease susceptibility and subject to reprogramming by enforced tolerance. Disease-associated microbiota may thus play a pathogenic role in food allergy.

SUBMITTER: Noval Rivas M

PROVIDER: S-EPMC3860814 | biostudies-literature | 2013 Jan

REPOSITORIES: biostudies-literature

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Publications

A microbiota signature associated with experimental food allergy promotes allergic sensitization and anaphylaxis.

Noval Rivas Magali M Burton Oliver T OT Wise Petra P Zhang Yu-qian YQ Hobson Suejy A SA Garcia Lloret Maria M Chehoud Christel C Kuczynski Justin J DeSantis Todd T Warrington Janet J Hyde Embriette R ER Petrosino Joseph F JF Gerber Georg K GK Bry Lynn L Oettgen Hans C HC Mazmanian Sarkis K SK Chatila Talal A TA

The Journal of allergy and clinical immunology 20121130 1

<h4>Background</h4>Commensal microbiota play a critical role in maintaining oral tolerance. The effect of food allergy on the gut microbial ecology remains unknown.<h4>Objective</h4>We sought to establish the composition of the gut microbiota in experimental food allergy and its role in disease pathogenesis.<h4>Methods</h4>Food allergy-prone mice with a gain-of-function mutation in the IL-4 receptor α chain (Il4raF709) and wild-type (WT) control animals were subjected to oral sensitization with ...[more]

PMID: 23201093

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Dataset Information

A microbiota signature associated with experimental food allergy promotes allergic sensitization and anaphylaxis.

Background

Objective

Methods

Results

Conclusion

Publications

A microbiota signature associated with experimental food allergy promotes allergic sensitization and anaphylaxis.

Similar Datasets

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