Measuring the energetics of membrane protein dimerization in mammalian membranes.
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ABSTRACT:
SUBMITTER: Chen L
PROVIDER: S-EPMC3860826 | biostudies-literature | 2010 Mar
REPOSITORIES: biostudies-literature
Publications
Measuring the energetics of membrane protein dimerization in mammalian membranes.
Chen Lirong L Novicky Lawrence L Merzlyakov Mikhail M Hristov Tihomir T Hristova Kalina K
Journal of the American Chemical Society 20100301 10
Thus far, quantitative studies of lateral protein interactions in membranes have been restricted peptides or simplified protein constructs in lipid vesicles or bacterial membranes. Here we show how free energies of membrane protein dimerization can be measured in mammalian plasma membrane-derived vesicles. The measurements, performed in single vesicles, utilize the quantitative imaging FRET (QI-FRET) method. The experiments are described in a step-by-step protocol. The protein characterized is t ...[more]
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