Ontology highlight
ABSTRACT:
SUBMITTER: Wong SQ
PROVIDER: S-EPMC3861801 | biostudies-literature | 2013
REPOSITORIES: biostudies-literature
Wong Stephen Q SQ Li Jason J Salemi Renato R Sheppard Karen E KE Do Hongdo H Tothill Richard W RW McArthur Grant A GA Dobrovic Alexander A
Scientific reports 20131213
Massively parallel sequencing offers the ability to interrogate a tumour biopsy for multiple mutational changes. For clinical samples, methodologies must enable maximal extraction of available sequence information from formalin-fixed and paraffin-embedded (FFPE) material. We assessed the use of targeted capture for mutation detection in FFPE DNA. The capture probes targeted the coding region of all known kinase genes and selected oncogenes and tumour suppressor genes. Seven melanoma cell lines a ...[more]