Ontology highlight
ABSTRACT:
SUBMITTER: Garg A
PROVIDER: S-EPMC3865775 | biostudies-literature | 2013 Nov
REPOSITORIES: biostudies-literature
Garg Ashish A Khosla Chaitan C Cane David E DE
Journal of the American Chemical Society 20131029 44
Incubation of [2-(2)H]-(2S,3R)-2-methyl-3-hydroxypentanoyl-SACP ([2-(2)H]-1a) with the epimerizing ketoreductase domain EryKR1 in the presence of a catalytic amount NADP(+) (0.05 equiv) resulted in time- and cofactor-dependent washout of deuterium from 1a, as a result of equilibrium isotope exchange of transiently generated [2-(2)H]-2-methyl-3-ketopentanoyl-ACP. Incubations of [2-(2)H]-(2S,3S)-2-methyl-3-hydroxy-pentanoyl-SACP with RifKR7 and with NysKR1 also resulted in time-dependent loss of d ...[more]