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Transcription factor beacons for the quantitative detection of DNA binding activity.

ABSTRACT: The development of convenient, real-time probes for monitoring protein function in biological samples represents an important challenge of the postgenomic era. In response, we introduce here "transcription factor beacons," binding-activated fluorescent DNA probes that signal the presence of specific DNA-binding activities. As a proof of principle, we present beacons for the rapid, sensitive detection of three transcription factors (TATA Binding Protein, Myc-Max, and NF-?B), and measure binding activity directly in crude nuclear extracts.

SUBMITTER: Vallee-Belisle A

PROVIDER: S-EPMC3866037 | biostudies-literature | 2011 Sep

REPOSITORIES: biostudies-literature

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Transcription factor beacons for the quantitative detection of DNA binding activity.

Vallée-Bélisle Alexis A Bonham Andrew J AJ Reich Norbert O NO Ricci Francesco F Plaxco Kevin W KW

Journal of the American Chemical Society 20110812 35

The development of convenient, real-time probes for monitoring protein function in biological samples represents an important challenge of the postgenomic era. In response, we introduce here "transcription factor beacons," binding-activated fluorescent DNA probes that signal the presence of specific DNA-binding activities. As a proof of principle, we present beacons for the rapid, sensitive detection of three transcription factors (TATA Binding Protein, Myc-Max, and NF-κB), and measure binding a ...[more]

PMID: 21815647

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