Dataset Information

Morphological and molecular characterization of Paramecium (Viridoparamecium nov. subgen.) chlorelligerum Kahl (Ciliophora).

ABSTRACT: We redescribe Paramecium chlorelligerum, a forgotten species, which Kahl (Tierwelt Dtl., 1935, 30:651) briefly but precisely described in the addendum to his ciliate monographs as a Paramecium with symbiotic green algae. The redescription is based on classical morphological methods and the analysis of the small subunit (SSU) rDNA. Morphologically, P. chlorelligerum differs from P. (C.) bursaria, the second green species in the genus, by having a special swimming shape, the length of the caudal cilia, the size of the micronucleus, the size of the symbiotic algae, the contractile vacuoles (with collecting vesicles vs. collecting canals), and the number of excretory pores/contractile vacuole (1 vs. 2-3). The molecular investigations show that P. chlorelligerum forms a distinct branch distant from the P. (Chloroparamecium) bursaria clade. Thus, we classify P. chlorelligerum in a new subgenus: Paramecium (Viridoparamecium) chlorelligerum. The symbiotic alga belongs to the little-known genus Meyerella, as yet recorded only from the plankton of a North American lake.

SUBMITTER: Kreutz M

PROVIDER: S-EPMC3866650 | biostudies-literature | 2012 Nov-Dec

REPOSITORIES: biostudies-literature

ACCESS DATA

Publications

Morphological and molecular characterization of Paramecium (Viridoparamecium nov. subgen.) chlorelligerum Kahl (Ciliophora).

Kreutz Martin M Stoeck Thorsten T Foissner Wilhelm W

The Journal of eukaryotic microbiology 20120724 6

We redescribe Paramecium chlorelligerum, a forgotten species, which Kahl (Tierwelt Dtl., 1935, 30:651) briefly but precisely described in the addendum to his ciliate monographs as a Paramecium with symbiotic green algae. The redescription is based on classical morphological methods and the analysis of the small subunit (SSU) rDNA. Morphologically, P. chlorelligerum differs from P. (C.) bursaria, the second green species in the genus, by having a special swimming shape, the length of the caudal c ...[more]

PMID: 22827482

Similar Datasets

Project description:Neokeronopsis (Afrokeronopsis) aurea nov. subgen., nov. spec. was discovered in soil from the floodplain of a small river in the Krueger National Park, Republic of South Africa. Its morphology, ontogenesis, and 18S rDNA were studied with standard methods. Furthermore, we supplemented the data on N. (N.) spectabilis by reinvestigating the preparations deposited in the British Museum of Natural History. Neokeronopsis (Afrokeronopsis) aurea is a very conspicuous ciliate because it has an average size of 330 × 120 μm and is golden yellow due to the orange-coloured cytoplasm and citrine cortical granules. Further main characteristics include the semirigid body; the urostylid cirral pattern with a distinct corona of frontal and pseudobuccal cirri both originating from the midventral rows; multiple anterior fragmentation of dorsal kineties 1-3; multiple posterior fragmentation of kinety 3, commencing with an unique whirl of kinetofragments; three caudal cirri; an oxytrichid/cyrtohymenid oral apparatus with polystichad paroral membrane and buccal depression; a single oral primordium developing along the transverse cirral row; and an oxytrichid 18S rDNA. These peculiarities are used to establish the new oxytrichid family Neokeronopsidae, the new subgenus Afrokeronopsis, and the new species N. (A.) aurea. Further, these features confirm the CEUU hypothesis, i.e., convergent evolution of a midventral cirral pattern in urostylid and oxytrichid hypotrichs; additionally, N. (A.) aurea is the first (semi)rigid hypotrich with cortical granules and the second one with midventral rows, breaking the granule and flexibility dogmas. These and other observations show that the phylogeny of the hypotrichs is full of convergences. Thus, only a combined effort of classical and molecular phylogeneticists will provide the data needed for a natural classification. Based on the CEUU hypothesis, the molecular data, and literature evidence, we suggest that midventral oxytrichids should be ranked as distinct families; accordingly, we establish a further new family, the Uroleptidae, which forms a distinct clade within the oxytrichid molecular trees. Neokeronopsis is possibly related to Pattersoniella because it has the same special mode of forming the buccal cirri and possesses a buccal depression found also in Steinia, a close relative of Pattersoniella. The large size and conspicuous colour make N. (A.) aurea a biogeographic flagship possibly confined to Africa or Gondwana, while Neokeronopsis (N.) spectabilis (Kahl, 1932) is an Eurasian flagship.

Project description:Using standard methods, we studied the morphology and 18S rDNA sequence of some peritrich ciliates from tank bromeliads of Costa Rica, Jamaica, and Ecuador. The new genus Orborhabdostyla differs from Rhabdostyla by the discoidal macronucleus. Two species from the literature and a new species from Ecuadoran tank bromeliads are combined with the new genus: O. previpes (Claparède and Lachmann, 1857) nov. comb., O. kahli (Nenninger, 1948) nov. comb., and O. bromelicola nov. spec. Orborhabdostyla bromelicola is a slender species with stalk-like narrowed posterior half and operculariid/epistylidid oral apparatus. An epistylidid relationship is also suggested by the gene sequence. Vorticella gracilis, described by Dujardin (1841) from French freshwater, belongs to the V. convallaria complex but differs by the yellowish colour and the number of silverlines. The classification as a distinct species is supported by the 18S rDNA, which differs nearly 10% from that of V. convallaria s. str. Based on the new data, especially the very stable yellowish colour, we neotypify V. gracilis with the Austrian population studied by Foissner (1979). Vorticella gracilis forms a strongly supported phyloclade together with V. campanula, V. fusca and V. convallaria, while Vorticellides astyliformis and Vorticella microstoma branch in a separate, fully-supported clade that includes Astylozoon and Opisthonecta. The new genus Vorticellides comprises five small (usually < 60 ?m), barrel-shaped species with two epistomial membranes: V. aquadulcis (Stokes, 1887) nov. comb., V. astyliformis (Foissner, 1981) nov. comb., V. platysoma (Stokes, 1887) nov. comb., V. infusionum (Dujardin, 1841) nov. comb., and V. (Spinivorticellides) echini (King, 1931) nov. comb. Two of these species are redescribed in the present study: V. astyliformis and V. aquadulcis, which is neotypified with a Costa Rican population. Pseudovorticella bromelicola nov. spec. differs from the congeners by the location of the two contractile vacuoles and the number of silverlines.

Dataset Information

Morphological and molecular characterization of Paramecium (Viridoparamecium nov. subgen.) chlorelligerum Kahl (Ciliophora).

Publications

Morphological and molecular characterization of Paramecium (Viridoparamecium nov. subgen.) chlorelligerum Kahl (Ciliophora).

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure

Tweets