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Regulation of thalamocortical axon branching by BDNF and synaptic vesicle cycling.


ABSTRACT: During development, axons form branches in response to extracellular molecules. Little is known about the underlying molecular mechanisms. Here, we investigate how neurotrophin-induced axon branching is related to synaptic vesicle cycling for thalamocortical axons. The exogenous application of brain-derived neurotrophic factor (BDNF) markedly increased axon branching in thalamocortical co-cultures, while removal of endogenous BDNF reduced branching. Over-expression of a C-terminal fragment of AP180 that inhibits clathrin-mediated endocytosis affected the laminar distribution and the number of branch points. A dominant-negative synaptotagmin mutant that selectively targets synaptic vesicle cycling, strongly suppressed axon branching. Moreover, axons expressing the mutant synaptotagmin were resistant to the branch-promoting effect of BDNF. These results suggest that synaptic vesicle cycling might regulate BDNF induced branching during the development of the axonal arbor.

SUBMITTER: Granseth B 

PROVIDER: S-EPMC3868945 | biostudies-literature | 2013

REPOSITORIES: biostudies-literature

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Regulation of thalamocortical axon branching by BDNF and synaptic vesicle cycling.

Granseth Björn B   Fukushima Yuichi Y   Sugo Noriuki N   Lagnado Leon L   Yamamoto Nobuhiko N  

Frontiers in neural circuits 20131220


During development, axons form branches in response to extracellular molecules. Little is known about the underlying molecular mechanisms. Here, we investigate how neurotrophin-induced axon branching is related to synaptic vesicle cycling for thalamocortical axons. The exogenous application of brain-derived neurotrophic factor (BDNF) markedly increased axon branching in thalamocortical co-cultures, while removal of endogenous BDNF reduced branching. Over-expression of a C-terminal fragment of AP  ...[more]

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