Dataset Information

Development, alteration and real time dynamics of conjunctiva-associated lymphoid tissue.

ABSTRACT:

Purpose

Conjunctiva-associated lymphoid tissue (CALT) is thought to play a key role in initiating ocular surface related immune responses. This study was planned to get first profound insights into the function of CALT related to development, cellular dynamics and morphological alteration using a novel mouse model.

Methods

Expression and morphology of CALT were investigated using BALB/c mice kept under different housing conditions, after topical antigen-stimulation and following lymphadenectomy and splenectomy. Particles and bacteria were applied topically to study antigen-transport. Intravital visualization was performed using two-photon microscopy.

Results

Postnatal development and ultrastructure of CALT in the mouse is similar to humans. Topical antigen-challenge significantly alters CALT expression. Bacterial translocation is demonstrated via lymphoepithelium whereas cellular velocities within follicles were approximately 8 µm/min.

Conclusions

CALT in the mouse is an immunological interface of the ocular surface, featuring dynamic processes such as morphological plasticity, particle/bacteria transport and cellular migration.

SUBMITTER: Siebelmann S

PROVIDER: S-EPMC3869694 | biostudies-literature | 2013

REPOSITORIES: biostudies-literature

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Publications

Development, alteration and real time dynamics of conjunctiva-associated lymphoid tissue.

Siebelmann Sebastian S Gehlsen Uta U Hüttmann Gereon G Koop Norbert N Bölke Torsten T Gebert Andreas A Stern Michael E ME Niederkorn Jerry Y JY Steven Philipp P

PloS one 20131220 12

<h4>Purpose</h4>Conjunctiva-associated lymphoid tissue (CALT) is thought to play a key role in initiating ocular surface related immune responses. This study was planned to get first profound insights into the function of CALT related to development, cellular dynamics and morphological alteration using a novel mouse model.<h4>Methods</h4>Expression and morphology of CALT were investigated using BALB/c mice kept under different housing conditions, after topical antigen-stimulation and following l ...[more]

PMID: 24376530

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Dataset Information

Development, alteration and real time dynamics of conjunctiva-associated lymphoid tissue.

Purpose

Methods

Results

Conclusions

Publications

Development, alteration and real time dynamics of conjunctiva-associated lymphoid tissue.

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