Ontology highlight
ABSTRACT:
SUBMITTER: Gilchrist EJ
PROVIDER: S-EPMC3869819 | biostudies-literature | 2013
REPOSITORIES: biostudies-literature
Gilchrist Erin J EJ Sidebottom Christine H D CH Koh Chu Shin CS Macinnes Tanya T Sharpe Andrew G AG Haughn George W GW
PloS one 20131220 12
We have generated a Brassica napus (canola) population of 3,158 EMS-mutagenised lines and used TILLING to demonstrate that the population has a high enough mutation density that it will be useful for identification of mutations in genes of interest in this important crop species. TILLING is a reverse genetics technique that has been successfully used in many plant and animal species. Classical TILLING involves the generation of a mutagenised population, followed by screening of DNA samples using ...[more]