Ontology highlight
ABSTRACT:
SUBMITTER: Keane FM
PROVIDER: S-EPMC3871272 | biostudies-literature | 2013
REPOSITORIES: biostudies-literature
Keane Fiona M FM Yao Tsun-Wen TW Seelk Stefanie S Gall Margaret G MG Chowdhury Sumaiya S Poplawski Sarah E SE Lai Jack H JH Li Youhua Y Wu Wengen W Farrell Penny P Vieira de Ribeiro Ana Julia AJ Osborne Brenna B Yu Denise M T DM Seth Devanshi D Rahman Khairunnessa K Haber Paul P Topaloglu A Kemal AK Wang Chuanmin C Thomson Sally S Hennessy Annemarie A Prins John J Twigg Stephen M SM McLennan Susan V SV McCaughan Geoffrey W GW Bachovchin William W WW Gorrell Mark D MD
FEBS open bio 20131208
The protease fibroblast activation protein (FAP) is a specific marker of activated mesenchymal cells in tumour stroma and fibrotic liver. A specific, reliable FAP enzyme assay has been lacking. FAP's unique and restricted cleavage of the post proline bond was exploited to generate a new specific substrate to quantify FAP enzyme activity. This sensitive assay detected no FAP activity in any tissue or fluid of FAP gene knockout mice, thus confirming assay specificity. Circulating FAP activity was ...[more]