Determination of HER2 amplification status on tumour DNA by digital PCR.
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ABSTRACT:
SUBMITTER: Garcia-Murillas I
PROVIDER: S-EPMC3873285 | biostudies-literature | 2013
REPOSITORIES: biostudies-literature
Publications
Determination of HER2 amplification status on tumour DNA by digital PCR.
Garcia-Murillas Isaac I Lambros Maryou M Turner Nicholas C NC
PloS one 20131226 12
Determination of the presence of HER2 amplification by quantitative PCR has been challenging, in part due to chromosomal instability and identification of a robust a reference region. We assessed the potential of digital PCR for highly accurate assessment of DNA concentration with EFTUD2 as chromosome 17 reference probe. We assessed a HER2:EFTDU2 ratio by digital PCR assay in the microdissected DNA from 18 HER2 amplified and 58 HER2 non-amplified cancers. The HER2:EFTUD2 ratio had high concordan ...[more]
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