Unknown

Dataset Information

0

RBSP3 (HYA22) is a tumor suppressor gene implicated in major epithelial malignancies.


ABSTRACT: Chromosome 3p21.3 region is frequently (>90%) deleted in lung and other major human carcinomas. We subdivided 3p21.3 into LUCA and AP20 subregions and discovered frequent homozygous deletions (10-18%) in both subregions. This finding strongly implies that they harbor multiple tumor suppressor genes involved in the origin and/or development of major epithelial cancers. In this study, we performed an initial analysis of RBSP3/HYA22, a candidate tumor suppressor genes located in the AP20 region. Two sequence splice variants of RBSP3/HYA22 (A and B) were identified, and we provide evidence for their tumor suppressor function. By sequence analysis RBSP3/HYA22 belongs to a gene family of small C-terminal domain phosphatases that may control the RNA polymerase II transcription machinery. Expression of the gene was drastically (>20-fold) decreased in 11 of 12 analyzed carcinoma cell lines and in three of eight tumor biopsies. We report missense and nonsense mutations in tumors where RBSP3/HYA22 was expressed, growth suppression with regulated transgenes in culture, suppression of tumor formation in severe combined immunodeficient mice, and dephosphorylation of ppRB by RBSP3/HYA22, presumably leading to a block of the cell cycle at the G1/S boundary.

SUBMITTER: Kashuba VI 

PROVIDER: S-EPMC387347 | biostudies-literature | 2004 Apr

REPOSITORIES: biostudies-literature

altmetric image

Publications


Chromosome 3p21.3 region is frequently (>90%) deleted in lung and other major human carcinomas. We subdivided 3p21.3 into LUCA and AP20 subregions and discovered frequent homozygous deletions (10-18%) in both subregions. This finding strongly implies that they harbor multiple tumor suppressor genes involved in the origin and/or development of major epithelial cancers. In this study, we performed an initial analysis of RBSP3/HYA22, a candidate tumor suppressor genes located in the AP20 region. Tw  ...[more]

Similar Datasets

| S-EPMC2684631 | biostudies-literature
| S-EPMC3335376 | biostudies-literature
| S-EPMC3250125 | biostudies-literature
| S-EPMC3494016 | biostudies-literature
| S-EPMC419484 | biostudies-literature
| S-EPMC3253429 | biostudies-literature
2012-12-21 | E-GEOD-38257 | biostudies-arrayexpress
| S-EPMC7469547 | biostudies-literature
| S-EPMC5634876 | biostudies-literature
| S-EPMC3818169 | biostudies-literature