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The spatial orientation of Helicobacter pylori in the gastric mucus.

ABSTRACT: The highly motile human pathogen Helicobacter pylori lives deep in the gastric mucus layer. To identify which chemical gradient guides the bacteria within the mucus layer, combinations of luminal perfusion, dialysis, and ventilation were used to modify or invert transmucus gradients in anaesthetized Helicobacter-infected mice and Mongolian gerbils. Neither changes in lumen or arterial pH nor inversion of bicarbonate/CO2 or urea/ammonium gradients disturbed Helicobacter orientation. However, elimination of the mucus pH gradient by simultaneous reduction of arterial pH and bicarbonate concentration perturbed orientation, causing the bacteria to spread over the entire mucus layer. H. pylori thus uses the gastric mucus pH gradient for chemotactic orientation.

SUBMITTER: Schreiber S

PROVIDER: S-EPMC387367 | biostudies-literature | 2004 Apr

REPOSITORIES: biostudies-literature

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The spatial orientation of Helicobacter pylori in the gastric mucus.

Schreiber Sören S Konradt Manuela M Groll Claudia C Scheid Peter P Hanauer Guido G Werling Hans-Otto HO Josenhans Christine C Suerbaum Sebastian S

Proceedings of the National Academy of Sciences of the United States of America 20040325 14

The highly motile human pathogen Helicobacter pylori lives deep in the gastric mucus layer. To identify which chemical gradient guides the bacteria within the mucus layer, combinations of luminal perfusion, dialysis, and ventilation were used to modify or invert transmucus gradients in anaesthetized Helicobacter-infected mice and Mongolian gerbils. Neither changes in lumen or arterial pH nor inversion of bicarbonate/CO2 or urea/ammonium gradients disturbed Helicobacter orientation. However, elim ...[more]

PMID: 15044704

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