Ontology highlight
ABSTRACT:
SUBMITTER: Wu S
PROVIDER: S-EPMC3874170 | biostudies-literature | 2014 Jan
REPOSITORIES: biostudies-literature
Wu Shiying S Chen Yu Y Mao Guanzhong G Trobro Stefan S Kwiatkowski Marek M Kirsebom Leif A LA
Nucleic acids research 20131003 1
We have used model substrates carrying modified nucleotides at the site immediately 5' of the canonical RNase P cleavage site, the -1 position, to study Escherichia coli RNase P RNA-mediated cleavage. We show that the nucleobase at -1 is not essential but its presence and identity contribute to efficiency, fidelity of cleavage and stabilization of the transition state. When U or C is present at -1, the carbonyl oxygen at C2 on the nucleobase contributes to transition-state stabilization, and thu ...[more]