Microarray Analysis of mRNA and MicroRNA Expression Profile Reveals the Role of β -Sitosterol-D-glucoside in the Proliferation of Neural Stem Cell.

ABSTRACT: Neural stem cells (NSCs) are self-regenerating cells, but their regenerative capacity is limited. The present study was conducted to investigate the effect of β -sitosterol-D-glucoside (BSSG) on the proliferation of hippocampal NSCs and to determine the corresponding molecular mechanism. Results of CCK-8 assay showed that BSSG significantly increased NSC proliferation and the effectiveness of BSSG was similar to that of basic fibroblast growth factor and epidermal growth factor. mRNA expression profiling showed that 960 genes were differentially expressed after NSCs were treated with BSSG. Among the 960 genes, IGF1 is considered as a key regulatory gene that functionally promotes NSC proliferation. MicroRNA (miRNA) expression profiling indicated that 30 and 84 miRNAs were upregulated and d

SUBMITTER: Jiang LH 

PROVIDER: S-EPMC3874330 | biostudies-literature | 2013

REPOSITORIES: biostudies-literature