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Capillary electrophoresis for the analysis of glycosaminoglycan-derived disaccharides.

ABSTRACT: Capillary electrophoresis is a common technique used for glycosaminoglycan-derived disaccharide analysis because of its high resolving power, high separation efficiency, high sensitivity, short analysis time, and straightforward operation. CE coupled to laser-induced fluorescence (LIF) detection shows an approximately 100 times higher sensitivity than traditional UV detection at 232 nm. 2-Aminoacridone (AMAC) is a widely used fluorophore for labeling unsaturated disaccharides by deductive amination, which is one of the most important method of derivatization of disaccharides for CE-LIF detection. Outlined in this chapter is a protocol of analyzing glycosaminoglycan-derived disaccharides by CE-LIF with AMAC derivatization.

SUBMITTER: Chang Y

PROVIDER: S-EPMC3875140 | biostudies-literature | 2013

REPOSITORIES: biostudies-literature

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Capillary electrophoresis for the analysis of glycosaminoglycan-derived disaccharides.

Chang Yuqing Y Yang Bo B Weyers Amanda A Linhardt Robert J RJ

Methods in molecular biology (Clifton, N.J.) 20130101

Capillary electrophoresis is a common technique used for glycosaminoglycan-derived disaccharide analysis because of its high resolving power, high separation efficiency, high sensitivity, short analysis time, and straightforward operation. CE coupled to laser-induced fluorescence (LIF) detection shows an approximately 100 times higher sensitivity than traditional UV detection at 232 nm. 2-Aminoacridone (AMAC) is a widely used fluorophore for labeling unsaturated disaccharides by deductive aminat ...[more]

PMID: 23386338

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