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Slowing DNA translocation through a nanopore using a functionalized electrode.

ABSTRACT: Nanopores were fabricated with an integrated microscale Pd electrode coated with either a hydrogen-bonding or hydrophobic monolayer. Bare pores, or those coated with octanethiol, translocated single-stranded DNA with times of a few microseconds per base. Pores functionalized with 4(5)-(2-mercaptoethyl)-1H-imidazole-2-carboxamide slowed average translocation times, calculated as the duration of the event divided by the number of bases translocated, to about 100 ?s per base at biases in the range of 50 to 80 mV.

SUBMITTER: Krishnakumar P

PROVIDER: S-EPMC3875158 | biostudies-literature | 2013 Nov

REPOSITORIES: biostudies-literature

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Slowing DNA translocation through a nanopore using a functionalized electrode.

Krishnakumar Padmini P Gyarfas Brett B Song Weisi W Sen Suman S Zhang Peiming P Krstić Predrag P Lindsay Stuart S

ACS nano 20131029 11

Nanopores were fabricated with an integrated microscale Pd electrode coated with either a hydrogen-bonding or hydrophobic monolayer. Bare pores, or those coated with octanethiol, translocated single-stranded DNA with times of a few microseconds per base. Pores functionalized with 4(5)-(2-mercaptoethyl)-1H-imidazole-2-carboxamide slowed average translocation times, calculated as the duration of the event divided by the number of bases translocated, to about 100 μs per base at biases in the range ...[more]

PMID: 24161197

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