Sortase A-mediated chemoenzymatic synthesis of complex glycosylphosphatidylinositol-anchored protein.

ABSTRACT: Green fluorescent protein and a glycosylphosphatidylinositol (GPI) anchor containing the common core structure and a lipid chain were synthesized and then coupled together in the promotion of bacterial sortase A (SrtA), which was the first example for the synthesis of a full-size GPI-anchored protein by SrtA, demonstrating that this can be a generally useful method for GPI-anchored protein synthesis.

SUBMITTER: Wu Z 

PROVIDER: S-EPMC3876033 | biostudies-literature | 2013 Dec

REPOSITORIES: biostudies-literature