Sortase A-mediated chemoenzymatic synthesis of complex glycosylphosphatidylinositol-anchored protein.
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ABSTRACT: Green fluorescent protein and a glycosylphosphatidylinositol (GPI) anchor containing the common core structure and a lipid chain were synthesized and then coupled together in the promotion of bacterial sortase A (SrtA), which was the first example for the synthesis of a full-size GPI-anchored protein by SrtA, demonstrating that this can be a generally useful method for GPI-anchored protein synthesis.
SUBMITTER: Wu Z
PROVIDER: S-EPMC3876033 | biostudies-literature | 2013 Dec
REPOSITORIES: biostudies-literature
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