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Immunological characterization of the spike protein of the severe acute respiratory syndrome coronavirus.


ABSTRACT: Severe acute respiratory syndrome (SARS) is a novel infectious disease caused by the SARS-associated coronavirus (SARS-CoV). There are four major structural proteins in the SARS-CoV, including the nucleocapsid, spike, membrane, and small envelope proteins. In this study, two sets of truncated fragments of spike protein were generated, the first were approximately 210-bp nonoverlapping fragments and the second were overlapping segments of 750 to 900 bp. From these 23 fragments, we identified a fragment of 259 amino acids (amino acids 441 to 700) that is a major immunodominant epitope. This fragment was highly expressed, and the purified fragment C could detect all 33 SARS patient serum samples tested, collected from 7 to 60 days after the onset of fever, but had no reactivity with all 66 healthy human serum samples tested. Thus, fragment C of spike protein was identified as an immunodominant antigen and could be used for serological detection of SARS-CoV infection.

SUBMITTER: Lu L 

PROVIDER: S-EPMC387621 | biostudies-literature | 2004 Apr

REPOSITORIES: biostudies-literature

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Immunological characterization of the spike protein of the severe acute respiratory syndrome coronavirus.

Lu Liqun L   Manopo Ivanus I   Leung Bernard P BP   Chng Hiok Hee HH   Ling Ai Ee AE   Chee Li Lian LL   Ooi Eng Eong EE   Chan Shzu-Wei SW   Kwang Jimmy J  

Journal of clinical microbiology 20040401 4


Severe acute respiratory syndrome (SARS) is a novel infectious disease caused by the SARS-associated coronavirus (SARS-CoV). There are four major structural proteins in the SARS-CoV, including the nucleocapsid, spike, membrane, and small envelope proteins. In this study, two sets of truncated fragments of spike protein were generated, the first were approximately 210-bp nonoverlapping fragments and the second were overlapping segments of 750 to 900 bp. From these 23 fragments, we identified a fr  ...[more]

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