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Third generation gold nanoplatform optimized for radiation therapy.

ABSTRACT: We report the design and fabrication of third generation ultrasmall PEGylated gold nanoparticles based platform (AuRad™) optimized for applications in radiation therapy. The AuRad™ nanoplatform has the following key features: (I) surface coating of hetero-bifunctional-PEG with amine, carboxyl, methoxy functional groups, which make this a versatile nanoplatform to conjugate various moieties like fluorophores, peptides, drugs, radiolabels; (II) size that is optimized for longer circulation, higher tumor uptake and modulated clearance; (III) high radiation enhancement. We have synthesized ultrasmall 2-3 nm gold nanoparticles, followed by attachment of hetero-bifunctional PEG and further conjugation of fluorophore AlexaFlour 647 for optical imaging, with a stability of more than 6 months. Confocal bioimaging with HeLa cells showed robust uptake of biocompatible nanoparticles in cells. Irradiation experiments X-rays showed greater than 2.8-fold cell kill enhancement as demonstrated by clonogenic survival assays. The results indicate that AuRad nanoplatform can act as potential theranostic agent in radiation therapy.

SUBMITTER: Kumar R

PROVIDER: S-EPMC3877840 | biostudies-literature | 2013 Aug

REPOSITORIES: biostudies-literature

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Third generation gold nanoplatform optimized for radiation therapy.

Kumar Rajiv R Korideck Houari H Ngwa Wilfred W Berbeco Ross I RI Makrigiorgos G Mike GM Sridhar Srinivas S

Translational cancer research 20130801 4

We report the design and fabrication of third generation ultrasmall PEGylated gold nanoparticles based platform (AuRad<sup>™</sup>) optimized for applications in radiation therapy. The AuRad<sup>™</sup> nanoplatform has the following key features: (I) surface coating of hetero-bifunctional-PEG with amine, carboxyl, methoxy functional groups, which make this a versatile nanoplatform to conjugate various moieties like fluorophores, peptides, drugs, radiolabels; (II) size that is optimized for long ...[more]

PMID: 24392307

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Third generation gold nanoplatform optimized for radiation therapy.

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Third generation gold nanoplatform optimized for radiation therapy.

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